人本主义教育思想与体育教学方法改革

本文分析了在当前体育教学改革新形势下的体育教学方法中的一些问题,提出应在人本主义教育思想指导下,进行体育教学语法改革。     

Enhancive effect of N,N′-dinitrosopiperazine on inducing precancerous lesion on nasal and/or nasopharyngeal epithelia of TgN(p53mt-LMP1)/HT mice

Objective: To investigate the enhancive effect of N,N′-dinitrosopiperazine (DNP) on induced carcinogenesis in nasal and/or nasopharyngeal epithelia among TgN(p53mt-LMP1)/HT transgenic mice to examine the underlying mechanism for the development of nasopharyngeal carcinoma (NPC). Methods: TgN(p53mt-LMP1)/HT transgenic mice and the same strain of C₅₇BL/6J wild-type mice both at the age of 5 months were randomly divided into 2 groups in parallel, respectively, i.e., TgN(p53mt-LMP1)/HT cancerous lesion-inducing group (TI), TgN(p53mt-LMP1)/HT control group (TC), C₅₇BL/6J cancerous lesion-inducing group (CI), and C₅₇BL/6J control group (CC). TI and CI mice were treated only with DNP for 16 weeks, twice each week, while TC and CC mice were given the same volume of saline as controls. At the end of treatment, animals were sacrificed to collect epithelial tissue samples from nasal cavity and nasopharynx for pathohistological evaluation by haematoxylin and eosin (HE) staining and for determination on the expression of TRAF2, c-Jun, and p16 by immunohistochemistry. Results: Atypical hyperplasia was more significant in the samples of TI than in those of TC, CI, and CC, with the rates of lesions being 90%, 10%, 0, and 0 (P<0.01) respectively, though DNP was used alone in a much shortened inducing period at less dosage and without the use of carcinogenic promoter 12-O-tetradecanoylphorbol-13-acetate as usual. The expressions of tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) and c-Jun in these samples were significantly up-regulated in TI (P<0.01), while the expression of p16 was significantly lower in TI than in the other groups (P<0.01). Conclusion: TgN(p53mt-LMP1)/HT mice hold inherited constitutional defect in immune surveillance function, which can be aggravated by environmental carcinogens, such as DNP used even though in a much less strength. The enhanced carcinogenesis-inducing effect of DNP on TgN(p53mt-LMP1)/HT mice should be closely associated with abnormal signaling of activator protein-1 (AP-1) pathway, especially up-regulated expressions of TRAF2 and c-Jun, and down-regulated expression of p16.     

超导电感储能脉冲输出电路设计及其关键问题

针对电感脉冲输出容易产生高电压,而且一般的断路开关也不易快速切断大电流这两个问题,提出了用模块化技术和脉冲变压器降低断路开关的开断电流,用金属氧化锌阀片(MOV)保护超导电感上的过电压的技术方案.以超导电感脉冲输出的单模块电路为对象,重点分析了氧化锌避雷器(由MOV组成)的U1mA(MOV通过1 mA电流时两端的电压)对脉冲输出波形的影响,提出了U1mA的选择原则.同时讨论了断路开关的开断时间对脉冲输出的影响.实验验证了此单模块电路的脉冲输出效果.     

某高校大学生复发性口腔溃疡诱发因素调查研究

目的:调查研究某高校大学生复发性口腔溃疡的诱发因素,为大学生预防复发性口腔溃疡提出建议.方法:采用自编问卷进行调查,多阶段分层随机抽样抽取样本;发放问卷1 045份,回收有效问卷987份;使用EpiData软件建立数据库,使用SPSS16.0进行数据处理和统计分析,研究复发性口腔溃疡与各因素的关系.结果:研究发现抽烟(P=0.003)、免疫力下降因素(P=0.000)、心理因素(P=0.022)、饮食类型(P=0.000)、内分泌(P=0.000)、食用烧烤(P=0.000)、消化系统慢性疾病(P=0.000)和口腔卫生(P=0.013)与复发性口腔溃疡的发生有统计学意义.结论:抽烟、免疫力下降因素、心理因素、饮食类型、内分泌、食用烧烤、消化系统慢性疾病和口腔卫生等是复发性口腔溃疡的诱发因素.     

高校廉政文化建设的理论与实践

高校廉政文化是社会主义廉政文化的重要组成部分,反映了当代中国先进文化的价值取向.加强高校廉政文化建设是践行"三个代表"重要思想的具体体现,是净化高校发展环境的迫切需要,同时也是培养高素质人才的内在要求.当前,高校加强廉政文化建设,主要应立足于建设廉政精神文化、廉政制度文化和廉政物质文化.     

一种基于密度的数据流检测算法SWKLOF

总结目前数据流在线检测算法的优缺点,提出了一种新的数据流在线检测算法—SWKLOF。该算法采用滑动时间窗口对数据流进行封装,用k-距离进行剪枝,剔除大部分正常数据,对剩余疑似异常数据采用局部离群因子LOF(local outlier factor)进一步精确筛选。理论分析和实验结果表明该算法降低了时间复杂度,提高了检测准确性。     

牛蒡子炮制前后质量标志物的网络药理学研究

研究牛蒡子炮制前后质量标志物的药效作用靶点,构建“成分-靶点-疾病”作用网络,解释牛蒡子炮制机理。 通过PharmMapper数据库筛选牛蒡子炮制前后6个质量标志物成分的潜在作用靶点,通过STRING数据库构建靶点蛋白相互作用网络;采用Cytoscape软件构建“成分-靶点-疾病”网络图。牛蒡子炮制前后质量标志物成分可通过多靶点作用,在抗肿瘤、抗炎、心脏损伤保护、抗高血压等方面发挥作用,且各个成分的相关作用靶点相互交联,共同发挥多靶点药效作用。通过网络药理学方法揭示了牛蒡子炮制前后质量标志物的主要药效作用,为进一步深入揭示其炮制机理提供了科学依据。     

城市老旧小区老年人户外活动休息点分布量化研究

为解决城市老旧小区内老年人户外活动休息点建设中易受主观因素影响,且未能真实反映老年人群体需求,无法快速评估和预测休息点的分布问题,通过跟踪记录与问卷访谈的方法,研究了南京市鼓楼区9个老旧小区老年人户外步行活动的特征,提出最优步行距离的概念及其计算方法用于描述特定老年人群体户外活动的水平,对老年人群体的户外活动步行距离进行量化,并以此为基础建立社区老年人户外活动休息点的分布模型,对9个小区休息点的理想分布进行计算,结果表明,该模型有利于客观地评价城市老旧小区中老年人户外活动休息空间的建设状况,有利于快速发现建成小区中休息点分布的不足,有利于直观预测规划设计中的老年人户外休息空间配置水平。     

Preparation of the selenomethionine derivative of tabtoxin resistance protein

In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed in E. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native.     

The new sideway of CNTF signal transduction pathway

The action of ciliary neurotrophic factor (CNTF) on intercellular free Ca²⁺ concentrations [Ca²⁺]_i induced by glutamate (Glu) in primary cultured hippocampal neurons were detected with Fura2/AM, a Ca²⁺-sensitive fluorophore, and the morphological influence of G-protein on it was objected. Glu could induce rapid increase of [Ca²⁺]_i in hippocampal neurons. CNTF had no significant action on [Ca²⁺]_i in resting hippocampal neurons. However, after incubation of CNTF for 5 min, the increase of [Ca²⁺]_i in hippocampal neurons rapidly induced by Glu was inhibited. Pretussis toxin (PTX)-sensitive G protein could block the action. These results indicate that a new non-genomic rapid sideway might exist in the upper stream of CNTF signal transduction pathway, which was related to Ca²⁺ signal transduction. Keywords: